How to use Plant Preservative Mixture (PPM) ?

Plant Preservative Mixture (PPM)

Introduction : 

Plant Preservative Mixture (PPM) is a solution used for the preservation of plant tissue and samples in various laboratory and research settings. It is designed to prevent the growth of microorganisms such as bacteria, fungi, and algae, which can degrade or contaminate plant samples. PPM is particularly useful for preserving plant samples used in molecular biology, genetics, and other research applications.

Composition of PPM : 

PPM typically contains a combination of different active ingredients that help in inhibiting microbial growth. The specific composition of PPM may vary depending on the manufacturer, but common ingredients include:

  1. PPM-P (pH buffer): This component helps maintain the pH of the solution in the acidic range, which is unfavorable for many microorganisms.
  2. PPM-A (antimicrobial agents): These are typically a mixture of broad-spectrum antibiotics and antifungal agents, such as cefotaxime and amphotericin B, which can inhibit the growth of various bacteria and fungi.
  3. PPM-C (chelating agents): Chelating agents, like citric acid, help to sequester metal ions that can promote the growth of some microorganisms.
  4. PPM-S (sugar): Sugars like sucrose are often included to provide a carbon source for the preserved plant tissues, which can enhance their longevity.
  5. PPM-V (vitamin): Certain vitamins may be added to support the survival of the plant tissues.

PPM – Plant Preservative Mixture description : 

PPM™ prevents the germination of both bacteria and fungi spores and is less expensive than commonly used antibiotics.

Plant Cell Technologies makes this heat stable preservative or biocide for better plant culture and proliferation.

PPM is used to effectively prevent or reduce microbial contamination in plant tissue culture. The Mixture Medium will not inhibit callus production or seed germination.

Medium used for in vitro seed germination, callus proliferation and callus regeneration.



Benefits of Plant Preservative Mixture (PPM) : 


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